• 25 stations @ 4 points per station
• Question number varies
  • 1 question = 4 points
  • 2 questions = 2 points each, 4 points total
  • 4 questions = 1 point each, 4 points total
  • 8 questions = 0.5 points each, 4 points total
• Stations will be timed at 2.5 minutes each
• Once the lab practical is complete, you will have 15 minutes to go back to any station for review
• Date/Time/Place : See lab syllabus for date, time and place are at regularly scheduled lab time and room
• Answers will be recorded on forms provided by the instructor
• Bring two writing utensils, pencils with erasers are preferred
• Refer to the student code of conduct (handbook, syllabi, addenda) regarding cheating

Material Covered

Safety: Be able to identify and describe the use of various safety procedures and equipment in the lab

Microscopy: Be able to identify microscope parts and give the definitions of important microscopy principles

Stains:

• Define stain and give the two major types based on their chemistry
• Know the difference between Simple Stains, Differential Stains, and Special Stains
• Know what stain is used for the various staining procedures and why
• Define mordant
• Describe 2 problems that you might encounter while performing a staining procedure
  

Culture Techniques

• Be able to show or describe proper transfer techniques between different media
• Be able to show or describe proper streaking procedures to obtain isolated colonies on agar media
  

Microorganisms

• Be able to identify and describe organism growth and reactions on media of all types
• Be able to identify and describe physical factors (nutrient source, temperature, pH, oxygen, etc.) of microorganisms
• Be able to identify and explain specific tests to determine normal flora and pathogenic microorganisms
• Be able to interpret antimicrobial chemotherapy results on a Kirby Bauer test
  

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