BIOL 2421 Microbiology         Metabolism Worksheet             Name: _______________
Dr. Weis                                  (25 points)                          Due Date: ____________

1.      Define energy as it relates to matter and give the formula. Give the various forms/types of energy and draw the chemical structure for a high energy compound and list four specific uses of this high energy compound in a bacterial cell. Properly define calorie, exergonic, and endergonic reactions. (2)

2.      Properly define metabolism and name and explain the two major pathways. Name three requirements for metabolism. Name and draw with labels two specific bacterial cell components that are involved with metabolism. (1.5)

3.      Define enzyme based on its general chemical structure and function. Draw with labels the major parts of an enzyme. Define denature and explain four things that might cause denaturing of an enzyme. Give an example of a specific enzyme and give its unique function. (2)

4.      Explain competitive and noncompetitive enzyme inhibitors and give examples for each. (1.5)

5.      Regarding glycolysis (EM pathway): give the starting product, the location, 4 important steps and the “end” products. (2)

6.      Explain why there are two other pathways for carbohydrate catabolism (PPP and ED), give their location, use, and their possible “end” products of glycolysis. (2)

7.      Properly define anaerobic respiration and their enzyme systems that cause the bacteria to be labeled a facultative anaerobe; and name the two types of fermentation processes and their possible end products and how these end products may be used in industry. (2)

8.      Define phosphorylation and explain the similarities and differences between substrate level and oxidative processes as to where they occur and the end result. (1.5)

9.      Properly define aerobic respiration. Explain the TCA cycle regarding possible locations in prokaryotes vs eukaryotes, the requirements, substrates needed, and intermediaries produced. (2)

10.  Define redox reactions, name coenzymes involved and where they occur in bacteria. (1.5)

11.  Explain the location, general structure (components), and function (steps) of bacterial ETC. (1.5)

12.  Explain how lipids (triglycerides) are used in metabolic processes. (1)

13.  Explain how proteins are used in metabolic processes. (1)

14.  Give the function of the major macromolecule groups (CH2O, Fats, and Proteins) as they relate to bacterial biosynthesis and use. (1.5)

15. Relate metabolic reactions to specific tests that may be done in the lab by giving four specific examples of metabolic tests and their end results/products seen to confirma a positive reaction. (1)