Dr. Weis Microbial Genetics CWID-4: _______________
1. Regarding the Nucleic Acids: explain in detail the following by comparing what is similar and contrasting what is different between Eukaryotic and Prokaryotic nucleic acids with regards to: (3)
- Structure(s)
- Location(s)
- Number or type(s)
- Function(s)
- Base pairing rules
2. Regarding DNA replication: (2.5)
- Define semi-conservative replication
- List the major enzymes involved in DNA replication and their purpose
- Explain the what is meant by leading and lagging strands
- Define and explain the purpose of methylation
3. Define the following terms and explain how they are related: (2.5)
- Genotype
- Phenotype
- DNA code
- Codon
- Anticodon
4. List and Explain all the events of Transcription (2)
5. List and Explain all the events of Translation (2)
6. Regarding Ribosomes (3)
- Describe the source, chemical composition, and function
- Compare and contrast eukaryotic/prokaryotic subunits
- Name and explain the three tRNA binding sites and their location
- Name four chemotherapeutic agents whose effects target the bacterial ribosomes and how each of these specifically affects ribosomal and cellular function.
7. Regarding regulation of gene expression (4)
- Name all the parts of an operon and their function.
- Explain the difference between Positive and Negative Operon controls
- List the various "sub"-types of negative controlled Operons and explain
in detail how they work
8. Regarding
Mutations: Name four different (4) types, their causes, effects, and clinical
use. (2)
9. For Genetic Recombination (2.5)
- Define horizontal vs. vertical transfer
- Explain the processes of conjugation, transformation, and transduction
- Generally explain what is meant by a transposon and their use/importance
10. Define plasmid and give three examples and explain their use/importance.
(1.5)